Ibicuruzwa
Trimethylol aminomethane
Koresha
Intermedi Hagati ya fosfomycine, irashobora kandi gukoreshwa nkumuvuduko wihuta wibirunga, kwisiga (cream, amavuta yo kwisiga), amavuta yubutare, paraffin emulsifier, buffer biologiya.
Byakoreshejwe nka gaze ya aside, gutegura buffer, surfactant, emulsifier na yihuta.Irakoreshwa kandi muri synthes organique.
Uburyo bwo kubyaza umusaruro
Kuramo Tris hamwe n'amazi make y'amazi abiri (300-500ml), ongeramo HCl, uhindure pH kuri 7.6 hamwe na HCl (1N) cyangwa NaOH (1N), hanyuma wongere wongere amazi abiri kuri 1000ml.Aya mazi namazi yabitswe, abitswe muri firigo kuri 4 ℃.
Icyitonderwa: Agaciro PH ya Tris-Hcl iratandukanye nubushyuhe, bityo rero igomba gupimwa mubushyuhe bwicyumba, ibisubizo byapimwe rero byizewe.
Buffering biranga
Tris ni intege nke hamwe na pKa ya 8.1 mubushyuhe bwicyumba (25 ℃).Ukurikije inyigisho ya buffering, urwego rwiza rwa tris buffers iri hagati ya pH7.0 na 9.2.
PH yumuti wamazi wa Tris base ni nka 10.5, kandi buffer yagaciro ka pH irashobora kuboneka wongeyeho aside hydrochloric kugirango uhindure agaciro ka pH kubiciro byifuzwa.Ariko, hakwiye kwitonderwa ingaruka zubushyuhe kuri pKa ya Tris.Impamyabumenyi, agaciro ka PH kagabanutseho 0.03.
1M Tris-HCl 6.8 na 1.5M Tris-HCl 8.8 nizo reagent zikoreshwa cyane kuri SDS-PAGE.
Mugihe TAE, TBE nizindi reagent zashizwe muri Tris nizo reagent zikoreshwa cyane muri ADN ya electrophoreis, TE (pH8.0) ikoreshwa cyane cyane mu gusesa ADN.(TE ni Tris wongeyeho EDTA.)
Tris buffers ntabwo ikoreshwa cyane nkumuti wa acide nucleic na proteyine, ariko kandi ifite akamaro gakomeye.Tris ikoreshwa mukuzamura poroteyine kristu mubihe bitandukanye bya pH.Imbaraga nkeya ionic ya Tris buffer irashobora gukoreshwa mugukora fibre intera intera ya lamin muri C. elegans.Tris kandi nikimwe mubice byingenzi bigize poroteyine electrophoreis.Byongeye kandi, Tris ni intera mugutegura surfactants, umuvuduko wihuta, hamwe nibiyobyabwenge.Tris nayo ikoreshwa nkibisanzwe.
Muburyo bwamashusho ninyandiko, irashobora gushiramo amakuru yibicuruzwa, serivisi nibyiza.Irashobora kurenga kubicuruzwa kugirango wongere ibintu bimwe (ibibazo byabakiriya, impungenge, nibindi), kurengera ibidukikije, ingero zubusa, nibindi
1. Ingero zapimwe kubuntu kugirango harebwe imikoreshereze isanzwe yabakiriya.
2. Ukurikije ibyifuzo byabakiriya batandukanye, ntoya kugeza 100g, nini kugeza kuri toni ya barrale, irashobora kuzuza ibisabwa byo gupakira.
3. Amasezerano yo kwishyura byoroshye, kohereza telegraphiki cyangwa kwemerwa (byujuje ibisabwa byo kwakira)
4. Ubwikorezi bwihuse, umunsi umwe cyangwa ejobundi birashobora gutangwa, inzira yose yo gukurikirana amakuru y'ibikoresho, kugirango ukoreshe neza abakiriya.
5. Serivisi nziza nyuma yo kugurisha, ihangane kandi witonde kugirango ikemure ibibazo byose bishobora kubaho, nkibibazo byubuziranenge, gufatanya byimazeyo nabakiriya kugirango bakemure byimazeyo, ntukange inshingano no gusubiza gusa.
6. Itsinda ryiza, gukora neza nubumenyi bwumwuga bituma abakiriya batizera gusa ibicuruzwa byacu, ariko kandi bakumva ko ikipe yacu yizewe.
7. Uburenganzira bwo kohereza ibicuruzwa byigenga byubaka isoko mpuzamahanga nibiranga isi yose.
8. Inyangamugayo, ibaruwa ishingiye, imyaka 20 yamateka yisosiyete nicyubahiro cyiza, reka abakiriya barusheho koroherwa, inyungu zabo hamwe no gutsindira inyungu, ubufatanye bwinyangamugayo.
